• SETD7 Depletion Promotes White Fat Browning

  2026-08-30

  This study identifies SETD7 as a negative regulator of inguinal white adipose tissue thermogenesis and links its depletion to an Adcy7–Sirt1–CREB1 signaling axis. The findings provide a mechanistic framework for studying adipose browning and metabolic protection in obese mice, while also highlighting important limits for translation to human metabolic disease.

• Medroxyprogesterone Acetate in Decidualization Assays

  2026-08-29

  Explore how Medroxyprogesterone acetate and MPA can be used as receptor-aware perturbations in endometrial decidualization research. This guide translates vitamin D/VDR findings into practical assay decisions while clearly separating validated evidence from exploratory applications.

• Bradykinin B2 Receptors and Ileal Peristalsis

  2026-08-28

  Chan and Rudd showed that bradykinin suppresses the peristaltic reflex in the isolated guinea pig ileum through pharmacologically defined B2 receptors, rather than B1 receptors. The study establishes a quantitative ex vivo framework for linking kinin receptor signaling to intestinal propulsion and clarifies how agonist, antagonist, and comparator responses can be interpreted.

• CRISPR/Cas9 mRNA–gRNA Editing of LGMN

  2026-08-28

  This study developed a lipid nanoparticle strategy for co-delivering Cas9 mRNA and guide RNA to disrupt LGMN, a lysosomal protease associated with aggressive breast cancer behavior. The work links an in vitro transcription-based RNA production workflow with reduced lysosomal/autophagic degradation, migration, invasion, and experimental lung metastasis, while also highlighting the preclinical limitations of this approach.

• Cy5-UTP: Direct Fluorescent RNA Labeling

  2026-08-27

  Cy5-UTP, also called Cyanine 5-uridine triphosphate, is a fluorescent UTP analog for T7 RNA polymerase-mediated in vitro transcription RNA labeling. It enables direct visualization of labeled RNA at excitation and emission maxima of 650 and 670 nm without a separate staining step, subject to assay-specific incorporation and detection controls.

• SEMA3E, Beige Fat, and β-Catenin Thermogenesis

  2026-08-27

  The 2026 reference study identifies SEMA3E as a regulator of beige adipocyte differentiation and cold-induced thermogenesis in mice. Through genetic perturbation, adipose transplantation, mitochondrial respiration assays, RNA sequencing, and pathway rescue experiments, it links SEMA3E activity to controlled Wnt/β-catenin signaling and oxidative phosphorylation.

• HyperScribe Co-transcription mRNA Kit Plus: Assay Guide

  2026-08-26

  This scenario-based guide explains how capped, polyadenylated mRNA design affects cell viability, proliferation, cytotoxicity, and translation experiments. It shows where HyperScribe™ Co-transcription mRNA Synthesis Kit Plus (ARCA, T7), SKU K1406, can improve workflow standardization while distinguishing product-supported facts from laboratory recommendations.

• Bestatin Inhibition of Leucine Aminopeptidase

  2026-08-26

  The 1991 PNAS study used high-resolution X-ray crystallography to show how Bestatin, also known as Ubenimex, occupies the catalytic site of bovine lens leucine aminopeptidase. Its zinc-coordinating groups and substrate-like side-chain contacts support a structural model in which Bestatin resembles a tetrahedral intermediate, providing a mechanistic framework for interpreting aminopeptidase inhibition and related biochemical assays.

• DNase I (RNase-free) in Chemoresistance Research

  2026-08-25

  DNase I (RNase-free) provides a rigorous pre-analytic safeguard for RNA-based studies of colorectal cancer chemoresistance. This guide connects ribonuclease-free DNase I use with assay controls, lactate–ANTXR1 biology, and evidence-based workflow design.

• MPA in Translational Reproductive Biology

  2026-08-25

  Medroxyprogesterone acetate is more than a conventional progestin tool. Its progesterone receptor activity, glucocorticoid receptor engagement, and tissue-specific effects create a useful framework for connecting decidualization, renal epithelial signaling, and neurobiology. This article translates recent R-loop and cGAS-STING findings into practical, hypothesis-driven MPA study designs.

• Dacomitinib Workflows for ErbB Cancer Research

  2026-08-24

  Dacomitinib (PF-00299804) enables sustained pan-ErbB suppression for studying receptor signaling, resistance, apoptosis, and cell-cycle behavior. This workflow also shows how to connect validated ErbB pharmacology with the METTL17–mitochondrial ferroptosis axis without overstating an unproven colorectal cancer application.

• Protein A/G Magnetic Beads for Cell Assays

  2026-08-24

  Learn how Protein A/G Magnetic Beads, SKU K1305, can strengthen the mechanistic arm of cell viability, proliferation, and cytotoxicity studies without being mistaken for a direct viability reagent. This scenario-based guide covers assay design, immunoprecipitation controls, protocol parameters, data interpretation, and practical supplier selection.

• Novobiocin Susceptibility in Resistant Staphylococci

  2026-08-23

  This 2010 veterinary dermatology study compared mupirocin and Novobiocin activity against methicillin-susceptible and methicillin-resistant staphylococci from healthy dogs and dogs with superficial pyoderma. Its main practical contribution is showing that Novobiocin retained substantial in vitro activity against resistant isolates, although susceptibility varied by clinical population and did not establish clinical efficacy.

• InstaBlue Protein Stain Solution for tiRNA Studies

  2026-08-22

  InstaBlue Protein Stain Solution provides a rapid Coomassie Brilliant Blue protein stain workflow for connecting translation-inhibition experiments with protein-level evidence. This article explains how to interpret gel signals, design controls, and avoid confusing visualization with quantitative or mechanistic proof.

• Trichostatin A (TSA): Designing Better Epigenetic Assays

  2026-08-21

  Trichostatin A (TSA) is more than a broad HDAC inhibitor: it can serve as a reversible probe for distinguishing chromatin-mediated silencing from sequence-level failure. This guide connects TSA mechanism, assay controls, and synthetic-biology applications to improve cancer and epigenetic research workflows.

8760 records 3/584 page Previous Next 12345 下5页 Last page